Determination of Extended Spectrum Beta- Lactamase Frequency of Klebsiella Pneumoniae Strains Isolated from Urinary Tract Infections and Typing with Isoelectric Focusing Method

Extended spectrum beta-lactamase (ESBL) presence was investigated in Klebsiella pneumoniae strains which were the ethiological agents of urinary tract infections and typed with isoelectric focusing (IEF) method. The antibiotic sensitivities of 52 K. pneumoniae strains were determined by disc diffusion method and ESBL were found in 12 strains (23 %) by double disc synergy test. Minimum inhibitory concentration (MIC) values of ESBL (+) K. pneumoniae strains for ceftazidime (CAZ) and cefotaxime (CTX) were investigated by E-test and isoelectric points of ESBL enzymes were determined by polyacrilamide gel electrophoresis. When the strains’ sensitivity patterns were investigated for 3 generation cephalosporins and aztreonam, 6 strains had ESBL premise sign with at least one antibiotic. Band pattern which focused around pl 7.6 was determined in 11 of 12 strains by IEF method. MIC values were found 16 μg/mL and over for CAZ and 12 μg/mL and over for CTX in 5 of these strains. When pI’s and antibiotic susceptibility patterns were analysed, 5 of 12 isolates had a band and susceptibility patterns suggesting SHV-2.